HEPES CAS: 7365-45-9 | ||||
HEPES is used as biochemisty buffers. It is referred to as a second generation Good's buffer, which displays improved solubility and stability compared to traditional Good's buffers. | ||||
CAS NO: | 7365-45-9 | |||
EINECS NO | 230-907-9 | |||
Ingredients | 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid | |||
Content of active ingredients | â¥99.5% | |||
Grade | AR/ACS equivalent | |||
Molecular Formula | C8H18N2O4S | |||
Molecular Weight : | 238.3 | |||
Buffering Range | 6.8~8.2 | |||
Packaging | Pkg of 1Kg/10Kg/25Kg | |||
Storage | Room Temperature | |||
Items | Specification | |||
Apparence | White Powder or Crystal | |||
A (Color, 0.5M NaOH solution) | â¤20 | |||
Solubility (Turbidity,0.5M NaOH solution) | Clear | |||
Assay (Titration by HCI) | â¥99.5% | |||
Infrared Spectrum Complies | Conforms to Structure | |||
UV Absorbance 260mm 0.1M aqueous solution | absorbanceâ¤0.03 | |||
UV Absorbance 280mm 0.1M aqueous solution | absorbanceâ¤0.03 | |||
UV Absorbance 260mm 1.0M aqueous solution | absorbanceâ¤0.05 | |||
UV Absorbance 290mm 1.0M aqueous solution | absorbanceâ¤0.05 | |||
25°C | 10.6~11.6 | |||
Loss on drying(105â for 3 Hours) Wt% | â¤3.0% | |||
Water insoluble | â¤0.03% | |||
Heavy Metals Pb | â¤5ppm | |||
Iron Fe | â¤5ppm | |||
Chloride Cl | â¤5ppm | |||
Calcium Ca | â¤10ppm | |||
Usage | ||||
HEPES is used in various biological application such as electroresis, protein separation. It is a zwitterion buffer, in general to work with other buffers to provide a stable rage. | ||||