The test is a qualitative, solid se, double antibodies sandwich immunochromatograc assay .The faecal sample must be diluted in the dilution buffer that is supplied with the test. The test makes use of a highly specific antibody/antigen reaction to detect H. pylori antigen in stool samples. This test has been designed to detect H. pylori antigen in human fecal samples through visual interpretation of color development in the test device. The test device contains a membrane strip, which is pre-coated with anti-H. pylori antibody at the Test on (T) and goat anti-mouse antibody at the Control on (C). An anti-H. pylori antibody color particle conjugate pad is located at the end of the membrane. When H. pylori antigen is present in the patient’s fecal sample and dissolved in buffered sa mixture of colloidal gold conjugate and extracted sample moves along the membrane chromatogracally by capillary action. This mixture migrates to the Test region (T) and forms a visible he antibodies complex with the H. pylori antigen. If H. pylori are not present in the extracted sample, no visible color band will form at the Test region (T).
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