Tri - Line HIV 1,2,O Home Testing Kits Blood Specimen 4mm Cassette Private Test

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INTENDED USE: The HIV 1.2.O Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatograc immunoassay for the qualitative detection of antibodies to Human Immunodeficiency Virus (HIV) type 1, type 2 and subtype O in whole blood, serum or plasma to aid in the diagnosis of HIV infection. SUMMARY AND EXPLANATION OF THE TEST HIV (Human Immunodeficiency Virus) is the etiologic agent of Acquired Immune Deficiency Syndrome (AIDS). The virion is surrounded by a lipid envelope that is derived from the host membrane. Several viral glycoproteins are on the envelope. Each virus contains two copies of positive-sense genomic RNAs. HIV-1 has been isolated from patients with AIDS and AIDS-related complex, and from healthy people with high potential risk for developing AIDS.1 HIV-1 consists of Subtype M and Subtype O. Highly divergent strains of HIV-1 were first recognized in 1990 and grouped provisionally as Subtype O as this variation has similar glycoprotein markers to HIV-1 but a slight variation to the protein marker. Although rarely compared to HIV-1 and HIV-2, infections caused by Subtype O have so far been identified in Africa (Cameroon), France and Germany. HIV-2 has been isolated from West African AIDS patients and from seropositive asymptomatic individuals.2 HIV-1, HIV-2, and Subtype O all elicit immune responses.3 Detection of HIV antibodies in serum, plasma or whole blood is the most efficient and common way to determine whether an individual has been exposed to HIV and to screen blood and blood products for HIV.4 Despite the differences in their biological characters, serological activities and genome sequences, HIV-1, HIV-2, and Subtype O show strong antigenic cross-reactivity.5,6 Most HIV-2 positive sera can be identified by using HIV-1 based serological tests. The HIV 1.2.O Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test to qualitatively detect the presence of antibodies to HIV type 1, type 2, and/or Subtype O in whole blood, serum or plasma specimen Test Principle The HIV 1.2.O Rapid Test Cassette (Whole Blood/Serum/Plasma) is a qualitative, membrane based immunoassay for the detection of antibodies to HIV-1, HIV-2, and Subtype O in whole blood, serum or plasma. The membrane is pre-coated with recombinant HIV antigens in the test ons, T1 and T2. The T1 test re-coated with HIV-1 and Subtype O antigen and the T2 test re-coated with HIV-2 antigen. During testing, the whole blood, serum or plasma specimen reacts with HIV antigen coated particles in the test strip. The mixture then migrates upward on the membrane chromatogracally by capillary action and reacts with recombinant HIV antigen on the membrane in the test on. If the specimen contains antibodies to HIV-1 and/or Subtype O, or HIV-2, one colored appear in the test on; if the specimen contains antibodies to HIV-1 and/or Subtype O, and HIV-2, two colored l appear in the test on. Both indicate a positive result. If the specimen does not contain HIV-1, Subtype O, and/or HIV-2 antibodies, no colored appear in the test on indicating a negative result. To serve as a procedural control, a colored always appear in the control on indicating that proper volume of specimen has been added and membrane wicking has occurred. INTERPRETATION OF RESULTS POSITIVE: Two or three distinct colored ear. One ld always appear in the control on (C), and another one or two apparent colored hould appear in the test on(s) (T1 and/or T2). *NOTE: The intensity of the color in the test on (T1 and T2) will vary depending on the concentration of HIV antibodies present in the specimen. Therefore, any shade of color in the test on (T1 and/or T2) should be considered positive. NEGATIVE: One colored ars in the control region (C). No apparent colored ear in the test ons (T1 and T2). INVALID: Control s to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control ure. Review the procedure and repeat the test with a new test device. If the problem persists, discontinue using the test kit immediately and your local distributor. LIMITATION

PERFORMANCE STUDY The HIV 1.2.O Rapid Test cassette (Whole Blood/Serum/Plasma) has correctly identified specimens of seroconversion panel and has been compared to a leading commercial ELISA HIV test using clinical specimens. The results show that the relative sensitivity of the HIV 1.2.O Rapid Test cassette (Whole Blood/Serum/Plasma) is >99.9% and the relative specificity is 99.9%.