Crystal tuning forks have a fixed resonant frequency when subjected to vibration. The most frequent frequency tuning forks used by musicians today are 440 Hz. The tuning fork is mainly used for tuning the instrument, and is also used to test the patient's hearing, and also as a treatment tool in some special treatments. (eg sonic acupuncture)
Sonic acupuncture, a kind of acupuncture that resembles acupuncture, but does not use needles, but uses ultrasound to stimulate acupuncture. Also known as ultrasound therapy.
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