Preparation of tris glycine buffer

As an important solution in chemical analysis, the buffer solvent has an "honored" status in testing, such as titrimetry or tometry, because the acidity and alkalinity of the experimental solution are often required to be kept within a reasonable range and the variation should not be too large. If these conditions are to be met, they must be accomplished by adding the appropriate amount of buffer, otherwise the overall experimental process will be affected. As tris glycine prepared into electric pulse buffer is very common, many people also want to know how it is prepared, so the following specific to introduce you.

I. Preparation method

1、Preparation reagents: Tris base, glycine, SDS

2, formula content: 30.3 grams of Tris base, 188 grams of glycine, 10 grams of SDS

3、Preparation method: use double-distilled water to dissolve until it dissolves to 1 liter, then prepare a 10-fold concentrate, and dilute it 10-fold when you use it. The prepared solution can be used for protein electroresis and used repeatedly 3-5 times.

Second, the role of prepared electroresis buffer

Electroresis buffer is an important part of nucleic acid and protein gel electroresis system, and also a conductor in the electroresis field, which can maintain the value of the whole electroresis system and ensure that it will not change.

C. Precautions for preparation and use

1、The packaging of the prepared solvent and raw material samples should be labeled to clearly indicate the name, specification, quality and date to avoid confusion with other reagents of the same type, which will affect the preparation effect.

2、If the reagent is found to be unlabeled, a small sample can be weighed first to avoid contamination during the weighing process, and the spoon should be used to remove it from the bottle, not to grab it directly by hand. At the same time, unusable reagents should be properly disposed of and should not be dumped at will. If the reagents are lumped together, a clean glass rod without impurities can be used to mash them and then remove them.

3, the solution can be taken in a clean container, avoid directly sucking with a pipette into the original apparatus, the reagents taken out from the experimental bottle, not used up, can not be poured back into the original bottle, and at the same time pay attention to and wear protective work clothes and gloves to operate.

As a manufacturer of buffer solution, the products produced by Desheng not only have high purity and stable process, the company conducts professional training for each position from time to time, especially for the personnel who interface with the products, so we can serve you in all aspects in terms of professional knowledge and answers. If you are interested, welcome to click the website to consult details!