Recombinant Human BMP-2 Protein

Construction

A DNA sequence encoding human BMP-2 (NP_001191.1) was expressed with the C-terminal fused Fc region of human IgG1.

Source

Human

Expression Host

HEK293

QC Testing Purity

> 90 % as determined by SDS-PAGE

Bio Activity

Depending on its ability to induce alkatase production in ATDC-5 , the expected ED 50 effectiveness is 40-100ng/mL.

Endo:p>

< 0.1EU per μg of the protein as determined by the LAL method.

Molecular Mass

The recombinant human BMP-2 contains 352 amino acids with a predicted molecular weight of 39.8 kD.

Formulation

Lyolized from sterile PBS, 7.4. Normally 6 % - 8 % trehalose, mannitol are added as protectants before lyolization.

Stability & Storage

Samples are stable for up to 24 months from date of receipt at 4 ℃and the dissolved liquid can be stored at -20℃ for 6-12 months. Avoid repeated freeze-thaw cycles.

Background

BMP-2 belongs to the transforming growth factor-βsuperfamily of structurally related ns.Bmp-2 is a potent osteoinductive cytokine that can be associated with collagen Combined with synthetic hydroxyapatite and other bone conduction vectors to induce the formation of bone and cartilage. In addition to osteogenic activity, BMP-2 occurs in the heart It plays an important role in life and is expressed in many tissues such as lung, liver, spleen, prostate, ovary and small intestine. The functional form of BMP-2 is one. The 26 kDa protein consists of two identical 114 amino acid polypeptides (monomers) linked together by a single disulfide bond. each The BMP-2 monomer is expressed as the C-terminal part of the precursor peptide, which also contains a of 23 amino acids for secretion and a 259 amino acids Amino acid precursors. After dimerization of the precursor, the covalent bond between the propeptide (also a homodimer of the disulfide bond) and the mature BMP-2 ligand was furan egg White enzyme cleavage.