Alkaline Protease

Alkaease

I. Brief Introduction:

The Alkaease is a solid enzyme produced by submerged fermentation from Bacillus licheniformis according to the specification of GB/T23527-2009 (China). It is widely applied in medicine industry, silk degumming,leather treatment,detergent, animal feed, animal fecal treatment etc.

II. Definition of Activity:

One gram of solid Alkaease powder at 40°C and 10.5 hydrolyze casein in one minute to produce 1μg tyrosine is defined as one enzyme activity unit and marked with u/g.

III. Characteristics:

1. Alkaease can efficiently hydrolyze the protein into free amino acids.

2. Effective temperature: 20-65°C Effective 8-12

Optimum temperature: 40-50°C Optimum 9-11

IV. Specifications:

V. Application & Reference Dosage:

1. Application in Heparin Sodium:

(1).Add salt and alkali at earlier stage. The process is the same with salt dissolving.

(2).Rise the low temperature to 65°C and keep 1 hour.

(3).Cool down the temperature to 45°C and add 3g enzyme powder (100,000 u/g) for one piece of small intestine.

(4).Rise the temperature to 60°C after adding the enzyme and keep 4-6 hours.

(5).Filtering after rising the temperature to 80°C.

(6).Add Resin when the temperature is cooled down to 60°C after the filtering.

(7).The procedure of dissolving salt is different.

Adjust the dosage and the enzymolysis condition according to the quality of raw materials and the practical production conditions.

2. Application in Chondroitin

(1).Stir the enzyme well with water, and then add it into the materials.

(2).Add 0.4%-0.6% of enzyme powder during the whole process of enzymolysis dissolution. Add the enzyme separately by twice. Add 60-70% of the total enzyme in the front two thirds time and add the rest enzyme during the left time.

(3).Enzymolysis condition: Adjust the temperature to 45-60°C and at 10.5. Process time: 5-8 hours. Stir once every 30mins.

Adjust the dosage and the enzymolysis condition according to the quality of raw materials and practical production condition.

3. Pretreatment in Silk Degumming:

Pretreatment → Dehydration → acid treatment (add protease 18-24 u/ml and soap 0.75% at 48-50°C and 10.5) → Dehydration → skin tearing off → washing →Dehydration → fluffing → drying → fluffing

4. Application in Leather Depilation:

Raw Hides → washing → degreasing → alkali expansion → feather plucking → splitting → dealkalization → enzyme depilation → washing → pickling →tanning.

Features:

l Simplify production process and reduce production period.

l Improve end product quality, leather yield and reduce production cost.

l No pollution to the equipment, environment and leather.

l Environmental-friendly & bio-degradation

5. Application in detergent:

Decompose protein based stains like grass, blood, mucous, milk, meat, faecal material, human sweat and etc. into small, readily soluble peptides and amino acids which can be easily removed during washing process and thus improving the cleaning efficiency of detergents.

Add Alkaease in the toothpaste, toothpowder, Mouth Refreshner can contribute to tartar removing.

6. Application in animal feed:

Add to the feed formula or directly with the mixed feed. Improve the utilization rate of protein and lower feeding cost.

7. Application in animal fecal treatment:

Effectively reduce ammonia gas and other gas noxious gas and fecal stink in livestock housing, improve breeding envirement and enhance animal health.

VI. Storage:

Should be stored in a cool, dry and well-ventilated place; protect from direct sunlight, heat and dampness; avoid storing with poisonous and harmful substances.

VII. Shelf Life:

12 months at ordinary temperature.

VIII. Packaging:

20kg/bag, 25kg/bag or as clients’ requirements

IX. Technical Support and Service:

Xingtai Sinobest Biotech Co., Ltd. will provide technical support service in the whole process. Our professional technicians will guarantee every client to use this product under proper guide.

X. Precautions:

Enzyme preparations belong to protein, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged may cause minor irritation for skin, eyes or mucous membrane of nose, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.