One Step Hepetitis C Virus Antibody Test CasseteSpecimen : serum and PlasmaIntroduction Assayplus one step anti-HCV test is a lateral flow immunoassay device for the visual detection of hepatitis C antibodies (anti-HCV) in serum or plasma. It is used as an aid in the diagnosis of hepatitis C infection. The assay is based on the principle of double antigen sandwich immunoassay for determination of anti-HCV in serum or plasma. Purified recombinant antigens are employed to identify anti-HCV specifically. This one step test is very sensitive and only takes 20 minutes for the result to be read. Test results are read visually without any instrument. Assay principle Serum or plasma sample is used for the test. After a specimen is loaded to the device, the specific antibodies, HCV IgG in the specimen sample react to the gold-conjugated HCV antigen. As HCV antibody - HCV antigen-gold conjugated complex migrates along nitrolose membrane, anti-HCV antibody in the complex is captured by the test ed with recombinant HCV antigen causing a pink to deep pink band. The intensity of the band is variable depending on the amount of antibody present in the blood sample. A red control ld always develop on the test strip to indicate the proper performance.
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