Total Aflatoxin (AFT) ELISA Test Kit

Introduction

• The test kit is detecting for the quantitative analysis of Total Aflaal, feed, peanuts.
• The Total Aflainfect corn, peanuts, nuts and etc.

principle of test

The Total AflaLISA Test Kit is based on a competitive colorimetric ELISA assay.

The rest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target yme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the d to the

well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the on the plate wells, last, take caloration with TMB substrate. The samples absorbance value is an inverse relationship with AFT content. Lastly, compared with the standard curve can be concluded the AFT residues in sample.

Technique Data

• Kit sensitivity: 0.02ppb (ng/ml)
• reactive mode: 25℃, 30min～15min
• Detection Limits:

• Cross-reaction rate:

Aflarc;€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦.……….100%

Aflarc;€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦..…………….75%

Aflarc;€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦..……………….100%

Aflarc;€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦.97%

Aflarc;€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦â€¦..……………….30%

• Sample Recovery rate:

Cereal, Compound feed …………………………………..……. 85±15%

Peanut …………………………………….. 82±15%

Edible oil …………………………………….. 85±15%

Sauces, Wheat, Other feed …………………………….. 83±15%

Beer …………………………………….. 84±15%

Wine, soy, vinegar …………………………….. 87±15%