Human Immunoglobulin G,IgG ELISA Kit

FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.

Purpose

This kit allows for the determination of IgG concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human IgG level in the sample，use Purified Human IgG antibody to coat microtiter plate wells, make solid-se antibody, then add IgG to wells, Combined IgG antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulric acid solution and the color change is measured spectrotometrically at a wavelength of 450 nm. The concentration of IgG in the samples is then determined by comparing the O.D. of the samples to the standard curve.