How does ADPS, a chromogenic substrate, develop colors?

The chromogen substrate ADPS (N-ethyl-N - (3-sulfopropyl) -3-methoxyanium salt dihydrate), as a novel Trinder's reagent, plays an important role in the field of biochemical analysis and detection. It is often used as a chromogenic substrate for horseradish peroxidase (HRP) or peroxidase (POD) to determine the content of various biomolecules. How does it develop color as a chromogenic substrate?

The color mechanism of ADPS substrate is based on the principle of enzyme catalyzed reaction. Under the catalysis of HRP or POD, hydrogen peroxide (H2O2) participates in the coupled oxidation reaction of substrate ADPS. The reaction product is a quinone imine type substance, with a significant increase in absorbance at 500nm. This characteristic makes ADPS a high-quality chromogenic substrate suitable for the determination of many enzyme catalyzed reactions.

Taking the detection of glucose (Glu) as an example: In the detection of Glu, glucose is oxidized by glucose oxidase (GOD) to produce gluconic acid and hydrogen peroxide (H2O2), which are key substances in the reaction. In this step, H2O2 acts as a catalyst for the chromogenic substrate ADPS.

The coupled oxidation reaction between ADPS and hydrogen peroxide will result in a significant increase in absorbance at 500nm, which is proportional to the content of the detected biomolecules. Therefore, in blood glucose detection, by measuring changes in absorbance, we can determine the glucose content in the blood. This analytical method is extremely sensitive and accurate, and has been widely applied in fields such as medical diagnosis and biological research.

In addition, the high water solubility of ADPS substrate is also an advantage of its application. It can be mixed with other reagents and is suitable for different types of experimental reagent systems. At the same time, its chemical characteristics make it stable in different reaction systems, ensuring the reliability of experimental results.

From the above, it can be seen that the chromogenic principle of the chromogenic substrate ADPS is based on enzyme catalyzed reactions, using the quinone imines produced by its coupled oxidation reaction with hydrogen peroxide to determine the content of the detected substance. In various biological analyses, such as blood glucose detection, ADPS makes the test faster and more sensitive, while precise analysis ensures the accuracy of the results. As a production supplier of chromogenic substrates, Desheng can provide water-soluble and high-purity raw materials and reagents, with a complete range of product types, including more than ten types such as MAOS, TOOS, DA64, DA67, etc., for customers to choose from at a discounted price. If you are interested, you can click on the website to inquire about our purchase at any time!