Matrigengel Matrix GFR Phenol Red Free-082703

PRODUCT DESCRIPTION

Basement membranes are continuous sheets of specialized extralar an interface between endothelial, epithelial, muscle, or neuronal and their adjacent stroma. Basement membranes are degraded and regenerated during development and wound healing. They not only support and layers, but they also play an essential role in tissue organization that affects adhesion, migration, proliferation, and differentiation. Basement membranes provide major barriers to invasion by metastatic tumor .

Wintop Matrigengel l Red Free) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor.Wintop Matrigengel l Red Free) at 37 °C to form a reconstituted basement membrane. The major components of Wintop Matrigengel l Red Free) include laminin, collagen IV, entactin, and heparin sulfate proteoglycan.

INTENDED USE

In addition to applications where Standard Matrigengel can be used，It is suitable for research applications where the preparation of basement membrane is highly demanding, such as the study of ion of tubular bone . It has also been used to study gene expression in primary mouse mammalian epithelial (reducing background by growth factors).

MATERIALS PROVIDED

PRODUCT PARAMETER

Source: Mouse Tumor

Appearance：

â‘ Color: nol-containing red matrigengel is yellow-pink, and nol-free red anslucent light yellow;

② Form: standard matrigengel dissolved at 4℃, a transparent liquid state; High concentration matrigengel after 0℃ solution, transparent liquid state, 4℃ for a long time to show a semi-gel.

Concentration: Protein concentration ranges from 8 to 26mg/mL

Endo‰¤ 4.5EU/ mL

Gel time: 5-30min gel at room temperature, the speed of gel formation is accelerated when the temperature is from 22°C to 37°C.

MATERIAL QUALIFICATIONS

l Routine screening of mouse colony pathogensby mouse antibody product (MAP) tests

l Testing for bacteria, fungi and mycoplasmato ensure negative results

l Extensive PCR testing for a variety of pathogens including LDEV to ensure strict control of raw materials used in the production process

l Extraction from LDEV-free mouse tumor

l Gel stability testing at 37℃ for 14 days

l Detection of endousing serological methods

l Biological function verification of each lot (organoid culture and differentiation experiments; Subcutaneous tumor formation test; Stem culture; Angiogenesis experiment etc.)

COATING PROCEDURES:

Thaw Matrigengel at 2-8 °C. Refrigerator temperatures may vary, therefore it is recommended to keep Matrigengel on ice in a refrigerator during the thawing process. Thawed Matrigengel solidifies quickly at temperatures above 15 °C; when working with Matrigengel,

keep it on ice to prevent untimely gelling.

There are many applications for Matrigengel which require different thicknesses and concentrations. A thick gel is needed for applications such as endothelial formation of capillary-like structures (Tube Formation Assay), the differentiation of rat aorta tissue into capillary-like structures (Aortic Ring Assay), epithelial organoid formation, or tumor organoid formation. Some applications, such as propagation of primary , require a thin layer coating and not a thick gel; therefore, the thin layer method should be used.

Thick Gel Method:

Thin Layer Method (non-gelling):