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Column chromatogra is the ideal method of chromatogra for purification and separation. It is a technique in which the stationary se is solid adsorbents like silica gel and activated alumina powder and the mobile se is a liquid. The principle of active compound separation depends on the activity of adsorbents and polarity of the solvent. If the polarity of the solvent is very low and the activity of the adsorbent is strong and high, then result of separation of compound is good. On the other hand, if the polarity of the solvent is very high and the activity of adsorbents is high then it gives poor results of compound separation. It means purification and isolation of compounds are not 100% pure.
| Size in Mesh | 30-70 | 60-120 | 60-200 | 70-230 | 100-200 | 230-400 |
| Unit  in microns (µm) | 210-500 | 125-250 | 74-250 | 63-210 | 74-149 | 40-63 |
| (10%  aqueous  solution) | 7+/-0.5 | 7+/-0.5 | 7+/-0.5 | 7+/-0.5 | 7+/-0.5 | 7+/-0.5 |
| Assay as SiO2 | Min 97% | Min 97% | 97.98% | 97-98.3% | 97-98.3% | 97-99.5% |
| Particle size above+In BetweenBelow- | 5% | 5% | 5% | 5% | 5% | 5% |
| 85% | 85% | 85% | Â 80% | 80% | 70% |
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