Mouse IL-3(Interleukin 3) ELISA Kit

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Synonyms: MCGF; MULTI-CSF; MCSF; M-CSF; Multiple; Multiolony-Stimulating Factor; Hematopoietic Growth Factor; P-Stimulating Factor; Mast-Growth Factor

IL-3(Interleukin 3) Basic information

Interleukin 3 (IL-3) is a protein that in humans is encoded by the IL3 gene localized on chromosome 5q31.1. Sometimes also called colony-stimulating factor, multi-CSF, mast growth factor, MULTI-CSF, MCGF; MGC79398, MGC79399: the protein contains 152 amino acids and its molecular weight is 17 kDa. IL-3 is produced as a monomer by activated T , monocytes/macroges and stroma . The major function of IL-3 cytokine is to regulate the concentrations of various blood-types. It induces proliferation and differentiation in both early pluripotent stem and committed progenitors. It also has many more specific effects like the regeneration of platelets and potentially aids in early antibody isotype switching. Interleukin 3 is an interleukin, a type of biological ) that can improve the body's natural response to disease as part of the immune system.[10] In conjunction with other β common chain cytokines GM-CSF and IL-5, IL-3 works to regulate the inflammatory response in order to clear pathogens by changing the abundance of various populations via binding at the interleukin-3 receptor.

Mouse IL-3(Interleukin 3) ELISA Kit test method

Feiyue’s Mouse IL-3(Interleukin 3) Elisa kit is an ELISA reagent for detection of IL-3(Interleukin 3) in Serum, plasma, tissue homogenates, lysates, culture supernates and other biological fluids

This kit uses sandwich ELISA to detect the concentRation of Mouse IL-3(Interleukin 3) -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse IL-3(Interleukin 3) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical by microplate reader. The concentRation of Mouse IL-3(Interleukin 3)in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1, GRCh38: Ensembl release 89: EN - Ensembl, May 2017

2, "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

3, "Entrez Gene: IL3 interleukin 3 (colony-stimulating factor, multiple)"