Minimum assay ( on dried material ) 98%Loss on drying 12.0 - 16.0%Melting point of isolated saccharin Not less than 226OCMaximum Limits of Impurities : Free acid 0.5 ml N%Free alkali 0.5 ml N%Arsenic ( As ) 0.0002%Lead ( Pb ) 0.001%Saccharin was used in high performance liquid chromatograc method for the simultaneous separation and determination of acesulfame potassium, saccharin and aspartame.