Human VWF(von Willebrand Factor) ELISA Kit

Feiyuebio's Human VWF(von Willebrand factor) ELISA Kit is an ELISA reagent for detection of VWF(Alpha Crosslaps) in Mouse serum, plasma or cell with sensitivity, specificity and consistency. VWF(Alpha Crosslaps) Introduction von Willebrand factor (VWF) (German: [fɔn ˈ vɪləbʁant]) is a blood glycoprotein involved in hemostasis, specifically, platelet adhesion. It is deficient and/or defective in von Willebrand disease and is involved in many other diseases, including thrombotic thrombocytopenic purpura, Heyde's syndrome, and possibly hemolytic–uremic syndrome. Increased plasma levels in many cardiovascular, neoplastic, metabolic (e.g. diabetes), and connective tissue diseases are presumed to arise from adverse changes to the endothelium, and may predict an increased risk of thrombosis.VWF is a large multimeric glycoprotein present in blood plasma and produced constitutively as ultra-large VWF in endothelium (in the Weibel–Palade bodies), megakaryocytes (α-granules of platelets), and subendothelial connective tissue.Von Willebrand Factor's primary function is binding to other proteins, in particular factor VIII, and it is important in platelet adhesion to wound sites. It is not an enzyme and, thus, has no catalytic activity. von Willebrand Factor has been shown to interact with Collagen, type I, alpha 1. Human VWF(von Willebrand factor) ELISA Kit Test method Product Name Human VWF(von Willebrand factor) ELISA Kit Catalog NO. FY-EH4400 Alias TNF-α ELISA Kit, Serum Amyloid A ELISA Kit Application VWF ELISA Kit allows for the in vitro quantitative determination of VWF concentrations in serum, plasma, tissue homogenates and other biological fluids. Size 48T, 96T Detection Method Sandwich ELISA, Double Antibody Storage 2-8 ℃ for 6 months Sensitivity ＜ 0.938 ng/ml Species Human UniProt ID P04275 CV %（） Intra-Assay: CV<8% Inter-Assay: CV<10% Note For Research Use OnlyThis kit uses sandwich ELISA to detect the concentration of VWF(von Willebrand factor) The microtiter plate provided in this kit has been pre-coated with an antibody specific to VWF(von Willebrand factor). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VWF(von Willebrand factor). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of VWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VWF(von Willebrand factor) in the samples is then determined by comparing the OD of the samples to the standard curve. Reference: 1,"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. 2, "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Previous: Human WISP1/CCN4(WNT1-inducible-signaling pathway protein 1) ELISA Kit Next: Human VEGFR2/KDR(Vascular endothelial growth factor receptor 2) ELISA Kit Wuhan Feiyue Biotechnology Co., LTD., is a professional supplier of biological research reagents such as ELISA kit, protein, antibody.                      Whatsapp: 08618062750658