Human PTEN ELISA Kit

A,Introduction Product Name Human PTEN (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN) ELISA Kit Catalog NO. FY-EH4232 Alias : PTEN Detection Method Sandwich ELISA, Double AntibodyApplication PTEN ELISA Kit allows for the in vitro quantitative determination of PTEN concentrations in serum, plasma, tissue homogenates and other biological fluids. Size : 96T Storage : 2-8℃ for 6 months Sensitivity : 0.094ng/ml Species : Human UniProt ID : P60484 CV（%） Intra-Assay: CV<8% Inter-Assay: CV<10% Note For Research Use Only Feiyue's Human PTEN (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN) ELISA Kit is an ELISA reagent for detection PTEN (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN) in Human serum, plasma or cell with sensitivity, specificity and consistency. Phosphatase and tensin homolog (PTEN) is a phosphatase, in humans, is encoded by the PTEN gene. Mutations of this gene are a step in the development of many cancers, specifically glioblastoma, lung cancer, breast cancer, and prostate cancer. Genes corresponding to PTEN (orthologs) have been identified in most mammals for which complete genome data are available. PTEN acts as a tumor suppressor gene through the action of its phosphatase protein product. This phosphatase is involved in the regulation of the cell cycle, preventing cells from growing and dividing too rapidly.[8] It is a target of many anticancer drugs. This kit uses sandwich ELISA to detect the concentration of PTEN (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN). The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTEN (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PTEN (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTEN(Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PTEN (Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN) in the samples is then determined by comparing the OD of the samples to the standard curve. B, Protocol Please contact: Tel: 08618062750658 Whatsapp/skype Reference: 1, ENSG00000284792 GRCh38: Ensembl release 89: ENSG00000171862, ENSG00000284792 - Ensembl, May 2017 2, GRCm38: Ensembl release 89: ENSMUSG00000013663 - Ensembl, May 2017 3, "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. 4, "Mouse PubMed Reference:". National Center for Biotechnolo