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Feiyue's Human IL-10 is an ELISA reagent for detection of IL-10 antibody, plasma or cell with
sensitivity, specificity and consistency.
Human IL-10(Interleukin 10) Introduction
IL-10 is an anti-inflammatory cytokine. IL-10 signals through a receptor complex consisting of two IL-10
receptor-1 and two IL-10 receptor-2 proteins. Consequently, the functional receptor consists of four IL-10 receptor molecules. IL-10 binding induces STAT3 signalling via the phosphorylation of
the cytoplasmic tails of IL-10 receptor 1 + IL-10 receptor 2 by JAK1 and Tyk2 respectively. The IL-10 protein is a homodimer; each of its subunits is 178-amino-acid long. IL-10 is classified as a
class-2 cytokine, a set of cytokines including IL-19, IL-20, IL-22, IL-24 (Mda-7), IL-26 and interferons type-I (IFN-alpha, -beta, -epsilon, -kappa, -omega), type-II (IFN-gamma) and
type-III.
Human IL-10(Interleukin 10) ELISA Kit Test Method
This kit uses sandwich ELISA to detect the concentration of Human IL-10.
Human IL-10-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.
Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IL-10 antibody with HRP-Streptavidin. TMB solution is
added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human IL-10 in the sample can be calculated by comparing the absorbance of the sample
with the standard curve.
Wuhan Feiyue Biotechnology Co., LTD., is a professional supplier of biological research reagents such as ELISA kit, protein, antibody.
Whatsapp: 08618062750658
