Recombinant Human IL-7 Protein

Product information A DNA sequence encoding the extracellular domain of human IL-7 (NP_000871.1) was expressed with the C-terminal fused Fc region of human IgG1. Human CHO cells > 95 % as determined by SDS-PAGE The ED50 was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2×106 units/mg. < 0.01EU per μg of the protein as determined by the LAL method The recombinant human IL-7 consists of 395 amino acids and predicts a molecular mass of 42.5 KDa. Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % trehalose, mannitol are added as protectants before lyophilization. Samples are stable for up to 24 months from date of receipt at 4 ℃ . Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.   Background IL-7, also known as interleukin 7, is a hematopoietic growth factor which belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. IL-7 stimulates the proliferation of lymphoid progenitors. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors, such as ConA and IL-2. It is important for proliferation during certain stages of B-cell maturation. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. IL7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL-7 is a hematopoietic growth factor that primarily affects early B and T cells.