Recombinant Human BMP-2 Protein

Construction A DNA sequence encoding human BMP-2 (NP_001191.1) was expressed with the C-terminal fused Fc region of human IgG1. Source Human Expression Host HEK293 cells QC Testing Purity > 90 % as determined by SDS-PAGE Bio Activity Depending on its ability to induce alkaline phosphatase production in ATDC-5 cells, the expected ED 50 effectiveness is 40-100ng/mL. Endotoxin < 0.1EU per μg of the protein as determined by the LAL method. Molecular Mass The recombinant human BMP-2 contains 352 amino acids with a predicted molecular weight of 39.8 kD. Formulation Lyophilized from sterile PBS, pH 7.4. Normally 6 % - 8 % trehalose, mannitol are added as protectants before lyophilization. Stability & Storage Samples are stable for up to 24 months from date of receipt at 4 ℃and the dissolved liquid can be stored at -20℃ for 6-12 months. Avoid repeated freeze-thaw cycles.   Background BMP-2 belongs to the transforming growth factor-βsuperfamily of structurally related signaling proteins.Bmp-2 is a potent osteoinductive cytokine that can be associated with collagen Combined with synthetic hydroxyapatite and other bone conduction vectors to induce the formation of bone and cartilage. In addition to osteogenic activity, BMP-2 occurs in the heart It plays an important role in life and is expressed in many tissues such as lung, liver, spleen, prostate, ovary and small intestine. The functional form of BMP-2 is one. The 26 kDa protein consists of two identical 114 amino acid polypeptides (monomers) linked together by a single disulfide bond. each The BMP-2 monomer is expressed as the C-terminal part of the precursor peptide, which also contains a signal sequence of 23 amino acids for secretion and a 259 amino acids Amino acid precursors. After dimerization of the precursor, the covalent bond between the propeptide (also a homodimer of the disulfide bond) and the mature BMP-2 ligand was furan egg White enzyme cleavage.