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Product information
Species Reactivity
Human
Specificity
Detects mouse Fcγ RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, no crossreactivity with recombinant mouse Fcrl3/CD162, rmFcγ RIA
or rhFcγ RIIA is observed
Source
CHO cells
QC Testing Purity
> 95 % as determined by SDS-PAGE
Purification
Protein A purified from cell culture supernatant
Immunogen
Recombinant human CD16A
Endotoxin Level
< 0.01EU per μg of the protein as determined by the LAL method
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with HSA
Stability & Storage
Samples are stable for up to 24 months from date of receipt at 4 ℃.
Avoid repeated freeze-thaw cycles.
Applications
1.ELISA(recommended concentration 1:2000-1:5000)
2.T Cell Stimulation: This antibody can be used to activate T cells when immobilized at
1-10 μg/mL (100 μL/well)
Background
Receptors for the Fc region of IgG (Fc γ Rs) are members of the Ig superfamily that function in the activation or inhibition of immune
responses such as degranulation, phagocytosis, ADCC (antibodydependent cellular toxicity), cytokine release, and B cell proliferation (13). The Fc γ Rs have been divided into three
classes based on close relationships in their extracellular domains; these groups are designated Fc γ RI (also known as CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). Each group may be
encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~108109M) capable of
binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~106107M) only recognizing IgG aggregates surrounding multivalent
antigens (1, 4). Fc γ Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosinebased activation motif (ITAM) within their cytoplasmic domains or associate
with one of the ITAMbearing adapter subunits, Fc Rγ or ζ (3, 5). The only inhibitory member in human and mouse, Fc γ RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosinebased
inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).
Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Iglike domains. It is expressed on a variety of myeloid and
lymphoid cells (4) and associates with Fc Rγ to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95%
amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to
regulate immune responses (5).