Bicine BUFFER 99.5% (CAS #150-25-4)

Bicine 99.5% (CAS #150-25-4) Bicine is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 7.6 – 9.0. It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, as well as a multiphasic buffer system for SDS-PAGE of proteins. It is soluble in water at 0°C up to 1.1 M. Bicine forms a complex with most common metals so stability constants and concentrations should be taken into consideration when choosing this buffer. CAS NO: 150-25-4 Ingredients N,N-Di(2-hydroxyethyl) glycine Purity ≥99.5% Grade AR/ACS Molecular Formula C6H13NO4 Molecular Weight : 163.17 g/mol Buffering Range 7.6-9.0 Packaging Pkg of 1Kg/10Kg/25Kg Storage Room Temperature  Items Specification  Apparence White Crystalline Powder Weight Conforms to claims Assay (Titration by NaOH)  ≥99.5% Infrared Spectrum Complies Conforms to standard reference Solubility(Color) Colorless Solubility(Turbidity) Clear UV abs@260nm ≤0.03 UV abs@280nm ≤0.03 UV abs@260nm ≤0.05 UV abs@290nm ≤0.05 pH@25°C 4.5~5.3 Water/loss on drying ≤0.5% Residue on ignition ≤0.1% Heavy Metals Pb ≤10PPM Iron,Fe ≤3PPM Sodium, Na ≤50PPM NH4 2- ≤20PPM Calcium,Ca ≤5PPM