HEPES Buffer 99.5% CAS: 7365-45-9

HEPES  CAS: 7365-45-9 HEPES is used as biochemisty buffers. It is referred to as a second generation Good's buffer, which displays improved solubility and stability compared to traditional Good's buffers. CAS NO: 7365-45-9 EINECS NO 230-907-9 Ingredients 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid Content of active ingredients ≥99.5% Grade AR/ACS equivalent Molecular Formula C8H18N2O4S Molecular Weight : 238.3 Buffering Range 6.8~8.2 Packaging Pkg of 1Kg/10Kg/25Kg Storage Room Temperature  Items Specification  Apparence White Powder or Crystalline APHA (Color, 0.5M NaOH solution)  ≤20 Solubility (Turbidity,0.5M NaOH solution) Clear Assay (Titration by HCI) ≥99.5% Infrared Spectrum Complies Conforms to Structure UV Absorbance 260mm 0.1M aqueous solution absorbance≤0.03 UV Absorbance 280mm 0.1M aqueous solution absorbance≤0.03 UV Absorbance 260mm 1.0M aqueous solution absorbance≤0.05 UV Absorbance 290mm 1.0M aqueous solution absorbance≤0.05 pH@ 25°C 10.6~11.6 Loss on drying(105℃ for 3 Hours) Wt% ≤3.0% Water insoluble ≤0.03% Heavy Metals Pb ≤5ppm Iron Fe ≤5ppm Chloride Cl ≤5ppm Calcium Ca ≤10ppm Usage HEPES is used in various biological application such as electrophoresis, protein separation. It is a zwitterion buffer, in general to work with other buffers to provide a stable pH rage.