BunnyMag Cell-free DNA Isolation Kit

BunnyMag Cell-free DNA Isolation Kit Prouduct Description Cell-free DNA (cfDNA) is a fragment of cfDNA released from cells into the bloodstream that can carry biological information from tumors, pathogens and fetuses. The analysis of cfDNA provides key markers for diagnosis, detection and monitoring of disease progression. Currently, this technology has been widely used in the fields of tumor mutation detection, targeted drug guidance and prognosis, pathogen detection and non-invasive prenatal screening. High quality nucleic acid is the guarantee for genomics research. BunnyTeeth Technology uses superparamagnetic bead purification technology to provide users with a highly sensitive nucleic acid extraction solution that easily achieves trace nucleic acid extraction for low concentration. As verified by thousands of clinical samples, this product can extract high quality cfDNA from cell-free body fluids such as fresh or frozen plasma, serum, pleural fluid, ascites and cerebrospinal fluid for scientific research and clinical in-vitro diagnostic use. Product Advantage  Efficient Extraction: High DNA extraction yield, good purity, effective removal of impurities and downstream PCR inhibitors. Flexible Sample Incorporation: Easy extraction of cfDNA from 0.5mL to 4mL samples with elution volumes as low as 20μL. Diverse Applications: Validated by clinical samples, designed for tumor, infection and prenatal diagnosis with liquid biopsy. Wide Support of Automated Platform: Compatible with manual and automated extraction procedures, support Tecan, Hamilton, PE and other nucleic acid extraction Experimental Data Figure 1. Efficient recovery of plasma cfDNA with different nucleic acid quantity. Addition of a known amount of exogenous DNA fragment to plasma, simulating 3 gradients of high and low nucleic acid content samples, and using 3 kits for simultaneous nucleic acid extraction. BunnyMag Cell-free DNA Isolation Kit can recover cfDNA fragments from samples with different nucleic acid quantity efficiently, and the nucleic acid yield and reproducibility are better than similar products from competing companies. Figure 2. Good linearity of cfDNA recovery efficiency in different volumes of plasma samples. The cfDNA yields showed good linearity with the plasma sample volume, using BunnyMag Cell-free DNA Isolation Kit for 0.5, 1, 2, and 4 mL plasma samples, respectively, and the peak patterns of the different volumes of extracted cfDNA were identical by Agilent 2100 DNA assay kit. Figure 3. cfDNA fluorescence quantitative PCR Ct values are the same as those of high-quality competing kits. Fluorescence quantification of 2 mL plasma samples after cfDNA extraction using different brands of cfDNA extraction kits, BunnyMag Cell-free DNA Isolation Kit performance is the same as top-class companies' products. Product Component Packing   Content Main components Lysis Binding Buffer Guanidine isothiocyanate, 5 M Washing Buffer 1 Guanidine isothiocyanate, 3 M Washing Buffer 2 Ethanol absolute, 80% Proteinase K 20 mg/mL Proteinase K Magnetic Beads Nano-magnetic beads Storage Conditions Please take out the magnetic beads and proteinase K and store at 2-8℃; others store at (10-30℃), the validity period is 12 months. Simple Workflow