Quick Details
- Processing Time:15-30days
- Port:TIANJIN, XIAMEN , GUANGZHOU , SHANGHAI ,QINGDAO
- Supply Ability: 1000000 Pieces Per Day
- Brand Name:BASEN
Diagnostic kit for anti-HAV-HEV IgM (Colloidal Gold)
PRODUCT DESCRIPTION
Brand :
BASEN
Intended Use:It is used for
diagnostic of anti-HAV-HEV IgM from human serum(or plasma) and can be used clinically.
Diagnostic Principles:The
anti-HAV-IgM kit depends on capture method and gold immunochromatography assay. The HAV antigen and mouse monoclonal antibody are coating in polyester fibre(or non-woven fibrics) and the test
lines with nitrocellulose membrane and control lines are coated with human IgMµ chain monoclonal antibody and IgG antibody separately. When the positive samples are added, the IgM antibody of HAV
virus in the serum of plasma can combined with the HAV-antigen in the colloidal gold. The compound can move forward due to the chromatography effect and form ‘Au-HAV Ag-HAV anti-human IgM Ab’
with pre-coated anti-human IgMµ chain monoclonal antibody when passing the testing lines. As a result, the lines turn to be amaranth. In the control lines, the free gold standard mouse monoclonal
antibody can attach to the anti-mouse IgG antibody and make the control lines amaranth. But in the negative group, the amaranth is only found in control lines.China Hav Test Cassette:BASEN
INDUSTRY CO.,LTD
The
anti-HEV-IgM kit depends on capture method and gold
immunochromatography assay. The HEV antigen and mouse monoclonal antibody are coating in polyester fibre(or non-woven fibrics) and the test lines with nitrocellulose membrane and control lines
are coated with human IgMµ chain monoclonal antibody and IgG antibody separately. When the positive samples are added, the IgM antibody of HEV virus in the serum of plasma can combined with the
HAV-antigen in the colloidal gold. The compound can move forward due to the chromatography effect and form ‘Au-HEV Ag-HEV anti-human IgM Ab’ with pre-coated anti-human IgMµ chain monoclonal
antibody when passing the testing lines. As a result, the lines turn to be amaranth. In the control lines, the free gold standard mouse monoclonal antibody can attach to the anti-mouse IgG
antibody and make the control lines amaranth. But in the negative group, the amaranth is only found in control lines.Hav Test Cassette Supplier:BASEN
Preserving Conditions and
Period of Validity:It is protected from light and dry place and is without frozen. The period of validity is 24 months from production. It can be stored in 65% humidity for one hour after it is
unsealed. It should be used immediately if the humidity is above 65%.
Sample Requirement:
1. The
venous blood should be sampled aseptically and separate the serum or plasma as soon as possible to avoid hemalysis.
2. The
fresh sample should be used. It can be stored in 2-8℃ for 7 days if the sample cannot be used immediately. It is be stored in -20℃ for long-time preservation and cannot be frozen more than 3
times.
3. 0.2mg/ml
heparin, 150mmol/L EDTA-2Na and 2.5% sodium citrate do no effect on the test result.
4. The
sample should not used if it is hemolytic.
5. 0.1%NaN3 has
no effect on the test result.
Testing Method:
You should read
the instruction thoroughly before using.
1. Take
the sample out to room temperature and number them.
2. Dilute
the serum or plasma 1:100 with dilution (take 2 ul sample to 198ul dilution and mixed thoroughly).
3. Take
the card out from the box, open the aluminum foil and put the card on the table.
4. Suction
the diluted serum or plasma with suction tube and put 2 drops to the pore.
5. Positive
samples can be tested in 1-15 minute