Quick Details
- Processing Time:3workdays
- Port:nanjing /shanghai
- Supply Ability: 500 Boxes Per Month
- Brand Name:parsbiochem
FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.
Purpose
This kit allows for the determination of IgG concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human IgG level in the sample,use Purified Human IgG antibody to coat microtiter plate wells, make solid-phase antibody, then add IgG to wells, Combined IgG
antibody which With HRP labeled,become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IgG
in the samples is then determined by comparing the O.D. of the samples to the standard curve.