Human CHEM（Chemerin）CLIA Kit

Catalog number: E-CL-H0514 ntended use This CLIA kit can be applied to the in vitro quantitative determination of Human CHEM concentrations in serum, plasma and other biological fluids. Test principle This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CHEM. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHEM and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHEM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CHEM. The concentration of Human CHEM in the samples can be calculated by comparing the RLU of the samples to the standard curve. Assay type Sandwich Format 96T Assay time 4.5h Reactivity Human Detection method Chemiluminescence Detection range 31.25-2000 pg/mL Sensitivity 18.75 pg/mL Sample volume 100μL Sample type Serum, plasma and other biological fluids Repeatability CV < 15% Kit Components& Storage The unopened kit can be stored at 4 ℃ for 1 week. If the kit is not used within 1 week, store the items separately according to the following conditions since the kit is received. Item Specifications Storage Micro CLIA Plate 8 wells ×12 strips -20℃, 6 months Reference Standard 2 vials Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months Biotinylated Detection Ab Diluent 1 vial, 14 mL HRP Conjugate Diluent 1 vial, 14 mL Concentrated Wash Buffer (25×) 1 vial, 30 mL Substrate Reagent A 1 vial, 5 mL 4℃ (shading light) Substrate Reagent B 1 vial, 5 mL Plate Sealer 5 pieces Product Description 1 copy Certificate of Analysis 1 copy Note:  All reagent bottle cap must be tighten to prevent evaporation and microbial pollution.  The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.