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Synonyms: MCGF; MULTI-CSF; MCSF; M-CSF; Multiple; Multilineage-Colony-Stimulating Factor; Hematopoietic Growth Factor; P-Cell Stimulating Factor; Mast-Cell Growth
Factor
Product name
Rat IL-3(Interleukin 3) ELISA Kit
Catalog NO.
FY-ER4752
Reactivity
Rat
Assay range
7.8-500pg/mL
Sensitivity
< 3.0
pg/mL
Alias
MCGF;
MULTI-CSF; MCSF; M-CSF; Multiple; Multilineage-Colony-Stimulating Factor; Hematopoietic Growth Factor; P-Cell Stimulating Factor; Mast-Cell Growth Factor
Detection Method
Sandwich ELISA,
Double Antibody
Size
48T, 96T
Uniprot ID
P04823
Storage
2-8 ℃ for 6
months
CV %()
Intra-Assay:
CV<8%
Inter-Assay:
CV<10%
Note
For Research Use
Only
IL-3(Interleukin 3) Basic information
Interleukin 3
(IL-3) is a protein that in humans is encoded by the IL3 gene localized on chromosome 5q31.1. Sometimes also called colony-stimulating factor, multi-CSF, mast cell growth factor, MULTI-CSF, MCGF;
MGC79398, MGC79399: the protein contains 152 amino acids and its molecular weight is 17 kDa. IL-3 is produced as a monomer by activated T cells, monocytes/macrophages and stroma cells. The major
function of IL-3 cytokine is to regulate the concentrations of various blood-cell types. It induces proliferation and differentiation in both early pluripotent stem cells and committed
progenitors. It also has many more specific effects like the regeneration of platelets and potentially aids in early antibody isotype switching. Interleukin 3 is an interleukin, a
type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. In conjunction with other β common chain cytokines GM-CSF and
IL-5, IL-3 works to regulate the inflammatory response in order to clear pathogens by changing the abundance of various cell populations via binding at the interleukin-3 receptor.
Rat IL-3(Interleukin 3) ELISA
Kit test method
Feiyue’s Rat IL-3(Interleukin 3) Elisa kit is an ELISA reagent for detection of IL-3(Interleukin 3)
in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
This kit uses sandwich ELISA to detect the concentRation of Rat IL-3(Interleukin 3)
-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex
is formed by additional anti-Rat IL-3(Interleukin 3) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The
concentRation of Rat IL-3(Interleukin 3) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
Reference:
4, Yang YC, Ciarletta AB, Temple PA, Chung MP, Kovacic S, Witek-Giannotti JS, Leary AC, Kriz R, Donahue RE, Wong GG (October 1986). "Human IL-3
(multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell. 47 (1): 3–10. doi:10.1016/0092-8674(86)90360-0. PMID 3489530. S2CID
37207637.
5, "IL3 (interleukin-3)". . Retrieved 2019-06-19.
6, Aiguo, Wu; Guangren, Duan (July 2006). "PMID Observer Design of Descriptor
Linear Systems". 2007 Chinese Control Conference. IEEE: 161–165. doi:10.1109/chicc.2006.4347343. ISBN 9787811240559. S2CID 72187.
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