Human IL-31(Interleukin-31) ELISA Kit

Synonyms: Interleukin-31; IL-31; IL31 Product name Human IL-31(Interleukin-31) ELISA Kit Catalog NO. FY-EH4753 Reactivity Human  Assay range 15.6-1,000pg/mL   Sensitivity ＜ 5.5 pg/mL Alias Interleukin-31; IL-31; IL31 Detection Method Sandwich ELISA, Double Antibody Size 48T, 96T Uniprot ID Q6EBC2 Storage 2-8 ℃ for 6 months CV %（） Intra-Assay: CV<8% Inter-Assay: CV<10% Note For Research Use Only IL-31(Interleukin-31) Basic information Interleukin-31 (IL-31) is a protein that in humans is encoded by the IL31 gene that resides on chromosome 12.  IL-31 is an inflammatory cytokine that helps trigger cell-mediated immunity against pathogens. It has also been identified as a major player in a number of chronic inflammatory diseases, including atopic dermatitis. IL-31 is produced by a variety of cells, namely type 2 helper (TH2) T-cells. IL-31 sends signals through a receptor complex made of IL-31RA and oncostatin M receptor β (OSMRβ) expressed in immune and epithelial cells. These signals activate three pathways: ERK1/2 MAP kinase, PI3K/AKT, and JAK1/2 signaling pathways.IL-31 is believed to play a role in chronic inflammation diseases. One of these diseases is atopic dermatitis, or eczema. When biopsy samples of patients with atopic dermatitis were compared to samples from patients without atopic dermatitis, levels of IL-31 were elevated in patients with atopic dermatitis. IL-31 plays a role in this disease by inducing chemokine genes CCL1, CCL17, and CCL22. The chemokines transcribed from these genes recruit T-cells to the irritated skin where they secrete more IL-31. This cycle is the current understanding of IL-31's role in atopic dermatitis. Along with atopic dermatitis, IL-31 is believed to play a role in inflammatory bowel disease and airway hypersensitivity.  Pruritic forms of inflammatory skin diseases, or itchy skin diseases, have been found to have elevated levels of IL-31 mRNA in patient biopsies. Analysis of the tissue distribution of the IL-31 receptor complex found that IL-31RA is abundant in dorsal root ganglia of different human tissues. Dorsal root ganglia is where the cell bodies of primary sensory neurons reside. Dorsal root ganglia are also believed to be where the "itch" sensation originates. These findings support the elevated levels of IL-31 in skin biopsies of pruritic skin diseases。 Human IL-31(Interleukin-31) ELISA Kit test method Feiyue’s Human IL-31(Interleukin-31) Elisa kit is an ELISA reagent for detection of IL-31(Interleukin-31) in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. This kit uses sandwich ELISA to detect the concentRation of Human  IL-31(Interleukin-31) -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human  IL-31(Interleukin-31）antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentRation of Human  IL-31(Interleukin-31）in the sample can be calculated by comparing the absorbance of the sample with the standard curve.   Reference: 1, GRCh38: Ensembl release 89: ENSG00000204671 - Ensembl, May 2017 2, "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.  3, "Entrez Gene: interleukin 31".