Catalog E-CL-H0514
This CLIA kit can be applied to the in vitro quantitative determination of Human CHEM concentrations in serum, plasma and other biological fluids.
This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CHEM. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CHEM and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CHEM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CHEM. The concentration of Human CHEM in the samples can be calculated by comparing the RLU of the samples to the standard curve.
| Assay type | Sandwich |
| Format | 96T |
| Assay time | 4.5h |
| Reactivity | Human |
| Detection method | Chemiluminescence |
| Detection range | 31.25-2000 pg/mL |
| Sensitivity | 18.75 pg/mL |
| Sample volume | 100μL |
| Sample type | Serum, plasma and other biological fluids |
| Repeatability | CV < 15% |
Kit Components& StorageThe unopened kit can be stored at 4 ℃ for 1 week. If the kit is not used within 1 week, store the items separately according to the following conditions since the kit is received.
Note: All reagent bottle cap must be tighten to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring. |
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